Table 2: The ultrafiltration centrifugation isolation method provided a high murine bronchoalveolar lavage fluid-derived extracellular vesicle yield.
The particle concentration and total particle count were measured by nanoparticle tracking analysis (NTA). The protein concentration and total amount of protein were measured by a bicinchoninic acid protein assay.
| Methods | Starting Volume (mL) | NTA* (x108/μL) | Total Particles† (x1010) | Protein Conc‡ (μg/μL) | Total Proteins§ (μg) |
|---|---|---|---|---|---|
| UFC | 35 | 7.69 ± 2.6 | 29.4 ± 18.4 | 3.7 | 3,136 ± 1860 |
| UC-DGC | 35 | 0.5 ± 0.05 | 0.5 ± 0.1 | 0.6 | 73.7 ± 38.3 |
*
BALF EVs’ particle concentration (mean ± SEM x 108/μL from three independent experiments).
†
BALF EVs’ total particle (mean ± SEM x 1010 particles from three independent experiments).
‡
BALF EVs’ protein concentration (mean ± SEM μg/μL from three independent experiments).
§
BALF EVs’ total protein (mean ± SEM mg from three independent experiments).
UFC: ultrafiltration centrifugation; UC-DGC: ultracentrifugation with density gradient centrifugation; Conc: concentration; NTA: nanoparticle tracking analysis; SEM: standard error of the mean.