Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Escherichia coli) | nuoe, nuof | GenBank | NC_012971.2 region2288438–2289174 | |
| Strain, strain background (Escherichia coli) | BL21-AI | Thermo Fisher Scientific Inc | C607003 | Chemically competent cells |
| Strain, strain background (Escherichia coli) | BL21FS | This study | BL21-AI supplemented with a twin-STREP-tag and a TEV protease recognition site on the nuoF N-terminus, see Materials and Methods,"Generation of an E. coli strain….” | |
| Recombinant DNA reagent | pCas (plasmid) | Jiang et al., 2015 | RRID:Addgene_62225 | A vector for Crispr-Cas9 mutagensis |
| Recombinant DNA reagent | pTargetF (plasmid) |
Jiang et al., 2015 | RRID:Addgene_62226 | A vector for Crispr-Cas9 mutagensis |
| Recombinant DNA reagent | pMSP2N2 (plasmid) |
Grinkova et al., 2010 | RRID:Addgene_29520 | A vector for expression of MSP2N2 membrane scaffold protein |
| Recombinant DNA reagent | DNA knock-in cassette | This study | NuoEF fragment supplemented with a twin-STREP-tag and a TEV protease recognition site, see Materials and Methods,"Generation of an E. coli strain….” | |
| Chemical compound, drug | Piericidin A | Cayman Chemical | 15379 | |
| Chemical compound, drug | NADH | Carl-Roth GmbH | AE12.2 | |
| Chemical compound, drug | Decylubiquinone; DQ | Sigma Aldrich BVBA | D7911 | |
| Chemical compound, drug | Ubiquinone-1; Q1 | Sigma Aldrich BVBA | C7956 | |
| Chemical compound, drug | Potassium ferricyanide (III); FeCy | Sigma Aldrich BVBA | 244023 | |
| Chemical compound, drug | Lauryl Maltose Neopentyl Glycol; LMNG | Anatrace | NG310 | |
| Chemical compound, drug | n-Dodecyl-β-D-Maltopyranoside; DDM | Anatrace | D310 | |
| Chemical compound, drug | E. coli polar extract | Avanti Polar Lipids | 100600C | |
| Software, algorithm | SerialEM 3.0.8 | Mastronarde, 2005 | RRID:SCR_017293 | |
| Software, algorithm | MotionCor2 | Zheng et al., 2017 | RRID:SCR_016499 | |
| Software, algorithm | CTFFIND-4.1 | Rohou and Grigorieff, 2015 | RRID:SCR_016732 | |
| Software, algorithm | crYOLO 1.4, crYOLO 1.7.0 | Wagner et al., 2019 | RRID:SCR_018392 | |
| Software, algorithm | Relion 3.1 | Zivanov et al., 2018 | RRID:SCR_016274 | |
| Software, algorithm | cryoSPARC 2.11, cryoSPARC 3.2.0 | Punjani et al., 2017 | RRID:SCR_016501 | |
| Software, algorithm | SWISS-MODEL server | Waterhouse et al., 2018 | RRID:SCR_018123 | |
| Software, algorithm | UCSF Chimera 1.13.1 | Pettersen et al., 2004 | RRID:SCR_004097 | |
| Software, algorithm | Coot 0.9 | Casañal et al., 2020 | RRID:SCR_014222 | |
| Software, algorithm | PHENIX 1.19.2 | Liebschner et al., 2019 | RRID:SCR_014224 | |
| Software, algorithm | ISOLDE 1.0b5 | Croll, 2018 | ||
| Software, algorithm | MolProbity | Williams et al., 2018 | RRID:SCR_014226 | |
| Software, algorithm | ConSurf server | Ashkenazy et al., 2016 | RRID:SCR_002320 | |
| Software, algorithm | UCSF ChimeraX 1.1 | Goddard et al., 2018 | RRID:SCR_015872 | |
| Software, algorithm | The PyMOL Molecular Graphics System, Version 2.4.1 | Schrödinger, LLC | RRID:SCR_000305 | |
| Other | Quantifoil R0.6/1 Cu300 holey carbon grids | Quantifoil | Q350CR-06 |