Table 5. Differences in the hydrogen bond network of iron-sulfur clusters in complex I structures solved at high resolution and water molecules in the immediate cluster environment.
(Only the clusters for which such comparison could have been done and clusters displaying differences in the environment are listed).
| Cluster, Subunit | Organism | ||
|---|---|---|---|
| E. coli this work | A. aeolicus [pdb: 6hla] |
O. aries [pdb: 6zk9] |
|
| N3, NuoF | His400 (+) | Leu395(-) | Leu407(-) |
| Trp363(-) | Glu349(+) | Gln361(+) | |
| Asn196(+) | His198(-) | Lys202(-) | |
| N1b, NuoG | HOH386 | HOH1070 | |
| N7, NuoG | HOH441 | ||
| HOH577 | |||
| Cys228 | Asp229 | ||
| Cys231 | Asp232 | ||
| Cys235 | Ser236 | ||
| Cys263 | Ser264 | ||
| HOH929* | |||
| HOH933* | |||
| HOH1005* | |||
| N4, NuoG | Thr203(+) | Val205(-) | |
| N5, NuoG | conserved | ||
| N6a, NuoI | Phe92(-) | Tyr109(+) | |
| HOH539 | |||
| N6b, NuoI | Leu48(-) | His65(+) | |
| Cys74(+) | Ala91(-) | ||
| Leu116(-) | Glu133(+) | ||
| N2, NouB | HOH438 | HOH353 | |
| HOH211 | HOH579 | ||
| Arg250D | Arg85 dimethylated | ||
| Ser62 | Ala53 | ||
*Water molecules replacing the N7 cluster.