Table 2.
S values (S(20,W)), frictional ratios (f/f0), and calculated molecular weights (including confidence intervals) of histone-DNA complexes derived from SV-AUC
| Sample | S(20,W) | f/f0 | Molecular weight (kDa) experimental/theoretical | |
|---|---|---|---|---|
| No GraFix | 147bp DNA | 5.10 (5.09, 5.10) | 3.09 (3.09, 3.09) | 117.13 (116.9, 117.3)/97 |
| MV-H2B-H2A 147 | 5.68 (4.61, 6.75) | 2.66 (2.51, 2.81) | 134.61 (89.83, 179.38)/150 | |
| MV-H4-H3 147 | 5.28 (4.59, 5.96) | 2.79 (2.70, 2.87) | 120.93 (104.14, 137.71)/146 | |
| MV-NLP147 | 7.78 (7.74, 7.82) | 2.0 (1.96, 2.04) | 179.17 (173.55, 185.80)/206 | |
| eNuc147 | 11.16 (11.15, 11.16) | 1.54 (1.52, 1.56) | 218.5 (214.4, 222.5)/210 | |
| GraFix | MV-NLP123 (nucleosome-like) | 9.86 (7.99, 11.72) | 1.77 (1.52, 2.02) | 221.46 (170.93, 271.99)/190 |
| MV-NLP147 (nucleosome-like) | 8.79 (7.30, 10.29) | 2.01 (1.86, 2.14) | 217.79 (140.57, 295.00)/206 | |
| MV-NLP207 (nucleosome-like) | 9.01 (7.48, 10.55) | 2.26 (2.00, 2.51) | 256.58 (194.54, 318.62)/245 | |