Table 1.
Cocktails of primary antibodies used for triple labelling immunohistochemistry
| Primary antibody | Dilution | Host | Company | Secondary antibody |
|---|---|---|---|---|
| QC | 1:100 | Goat | IZI | Donkey anti-goat Cy2 |
| α-Synuclein (Syn1) | 1:3000 | Mouse | BD transduction | Donkey anti-mouse Cy3 |
| TH | 1:200 | Guinea pig | Synaptic systems | Donkey anti-guinea pig Cy5 |
| QC | 1:100 | Goat | IZI | Donkey anti-goat Cy2 |
| pGlu79-α-synuclein | 1:100 | Rabbit | IZI | Donkey anti-rabbit Cy3 |
| α-Synuclein (Syn1) | 1:3000 | Mouse | BD transduction | Donkey anti-mouse Cy5 |
Secondary antibodies were all from Dianova and used at a dilution of 1:200
TH tyrosine hydroxylase, QC glutaminyl cyclase