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. 2021 Jun 14;142(3):475–494. doi: 10.1007/s00401-021-02325-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Good correlation between quantitative multiplexed phosphorylated Rab10^Thr73/total Rab10 immunoblotting and targeted pRab10^Thr73occupancy assays in peripheral blood neutrophils. a pRab10^Thr73 phosphorylation occupancies in DMSO treated neutrophils per individual arranged in descending order by group (b) and aligned quantitative immunoblotting analysis of pRab10^Thr73/total Rab10 ratios (b) were plotted as mean with SD for all 101 participants. c Pearson correlation between immunoblotting for pRab10^Thr73 phosphorylation/total Rab10 protein levels and targeted pRab10^Thr73 occupancies. Blotted are mean values of two independent experiments for each method (R² = 0.78, p < 0.0001)