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. 2021 Jun 14;142(3):475–494. doi: 10.1007/s00401-021-02325-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Representative results for five controls and five R1441G mutation carriers. a Immunoblotting of neutrophils isolated from five R1441G LRRK2 mutation carriers with PD (n = 2) and non-manifesting carrier status (n = 3) as well as five controls with duplicate loading of 10 µg of DMSO and MLi-2 (200 nM MLi-2, 30 min) treated whole cell extracts using antibodies against total LRRK2, pSer935 LRRK2, total Rab10, MJFF-pRAB10 (pThr73) and GAPDH. (b) Quantification of immunoblots of two independent experiments by analysing pRab10^Thr73 /total Rab10 ratio of DMSO treated samples b per individual and c per group with group difference calculated by unpaired t test (***p = 0.0007). d The respective summed intensities of fragment ion transitions are represented with different colours as shown in the bottom panel of the graph. e Relative endogenous pRab10 peak areas of two independent analysis for pRab10^Thr73 occupancy depicted as a bar graph for DMSO and MLi-2 treated samples of all ten participants and f per group with group difference calculated by unpaired t test (**p = 0.0014)