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. 2021 Aug 11;11:16299. doi: 10.1038/s41598-021-95286-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Unk^cKO mice have reduced Raptor levels in the developing nervous system. (A) Unkempt binds to Raptor but not Raptor mutant 4 in a Raptor overlay assay. HA-Raptor and HA-Raptor mutant 4 expression levels were determined by western blot (lower panel). (B) Western blot of Raptor expression in Unk^cKO E16.5 embryonic brain tissue. (C,D) Quantification of Unkempt and Raptor expression in Unk^cKO E16.5 embryonic brain tissue. (E) Western blot of rpS6, phospho-rpS6 (P-rpS6), 4E-BP2 and phospho-4E-BP (P-4E-BP) expression in Unk^cKO E16.5 embryonic brain tissue. (F–J) Quantification rpS6, P-rpS6, 4E-BP2, P-4E-BP and P-AKT expression in Unk^cKO E16.5 embryonic brain tissue. Data are represented as mean ± SEM. Student’s t test, *p < 0.05, ***p < 0.001, n.s. not significant. Control n = 3, Unk^cKO n = 3.