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. 2021 Aug 11;11:16348. doi: 10.1038/s41598-021-94855-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of spontaneous inflammation in 8-month-old transgenic (TG) mice. (a) mRNA levels of cytokines (IFN-α, TNF-α, IL-17 and IFN-γ) in the spleens of 5- and 8-month-old WT and TG mice measured by qRT-PCR. (b) Flow cytometric analysis of single cells from the spleens of 8-month-old mice. ((b), upper) A strategy to determine the population of monocytes, neutrophils, macrophages and dendritic cells is represented. To investigate the population of monocytes and neutrophils, CD11b⁺ cells were gated first. Ly6C⁺Ly6G⁺ cells were considered to be neutrophils, and Ly6C⁺Ly6G^- cells were considered to be monocytes. CD11b⁺F4/80⁺ cells served as macrophages, and CD11c⁺ cells represented neutrophils. ((b), lower) Monocyte, neutrophil, macrophage and dendritic cell populations are represented. (c) mRNA levels of cytokines IFN-α, TNF-α, IL-17 and IFN-γ, (d) mRNA expression levels of chemokines CCL2, CCL7, CCL12, CXCL1 and CX3CL1. (e) TLR7 mRNA expression levels in the kidneys of 8-month-old WT and TG mice, measured by qRT-PCR. WT, wild type. *p < 0.05; **p < 0.01, ***p < 0.001 versus WT group of the same age.