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. 2021 Aug 11;11:16287. doi: 10.1038/s41598-021-95705-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of in vitro protein expression by immunofluorescence (IF). (A) Radar plot showing in vitro effect of DHT on expression of LPAR1 (blue) or ALDH1A1 (orange) in non-DE eSCs with E2 ± DHT and in DE-eSCs with E2P4 ± DHT in both groups (Ctrl, PCOS; both n = 3). The y-axis shows log fold change (LFC) values calculated for ratios with and without DHT; LFC 0 line (no change in expression) is shown in dotted black. (B) IF panel for LPAR1 and ALDH1A1 protein expression in non-DE-eSCs with E2 or E2DHT post-treatment and DE-eSCs with E2P4 or E2P4DHT post-treatments in eSC_Ctrl and eSC_PCOS; nuclei are stained with DAPI. Scale bar: 100 µm.