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. 2021 Aug 11;7:212. doi: 10.1038/s41420-021-00572-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Surface markers of HucMSCs: CD73, CD90, and CD105 (positive markers), and CD31, HLA-DR, and CD34 (negative markers) were detected using flow cytometry. B Morphology of HucMSCs was observed under a light microscope (left); osteogenesis, adipogenesis, and chondrogenesis were observed using alizarin red staining, oil red O staining, and alcian blue staining respectively. C the ultrastructure of HucMSCs-EVs was observed by TEM. D the particle size of EVs was detected using Nanosight analysis. E the surface marker proteins of EVs (CD63, CD9, and TSG101) were detected using Western blotting. The experiment was repeated three times.