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. 2021 Aug 11;7:212. doi: 10.1038/s41420-021-00572-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A, B The transfection efficiency of PTEN adenovirus was detected using RT-qPCR and Western blotting. SCI rats were co-treated with HucMSCs-EVs and PTEN adenovirus. C Pathological changes were measured using HE staining. D Nissl bodies were detected using Nissl staining. E–G expressions of NeuN, GFAP, and NF200 were detected using immunohistochemistry. H Apoptosis in spinal cord tissues of SCI rats was detected using TUNEL. N = 6. Data are expressed as mean ± standard deviation and analyzed using and analyzed using t test or one-way ANOVA, followed by Tukey’s multiple comparisons tests, ***p < 0.001.