Table 1.
List of genes showing significant fold change in microarray.
| Gene | Gene name | Fold change (Absolute) KD vs CD | P-value |
|---|---|---|---|
| Upregulated | |||
| Mitocondrial associated genes | |||
| Coq3 | Hexaprenyldihydroxybenzoate methyltransferase (Synthesis of Coenzyme Q) | 1.8260276 | p = 0.055436 |
| Nd6 | NADH-ubiquinone oxidoreductase chain 6 (Complex I) | 1.2989544 | 0.028589 |
| Pdk4 | Pyruvate dehydrogenase kinase, isozyme 4 | 1.2670953 | 0.028164 |
| Cox11 | COX11 cytochrome c oxidase assembly homolog (yeast) (Assembly of Complex IV) | 1.243264 | 0.03916 |
| Bcl2 | B-cell CLL/lymphoma 2 | 1.2251029 | 0.009542 |
| Ndufa12 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (Complex I) | 1.2011336 | 0.014646 |
| Tomm40b | Translocase of outer mitochondrial membrane 40 homolog B (yeast) | 1.1915064 | 0.020061 |
| Mrps14 | Mitochondrial ribosomal protein S14 | 1.147669 | 0.015547 |
| Gcsh | Glycine cleavage system protein H (aminomethyl carrier) | 1.116142 | 0.029307 |
| Mrpl22 | Mitochondrial ribosomal protein L22 | 1.1113199 | 0.018437 |
| Ssbp1 | Single-stranded DNA binding protein 1 | 1.1083369 | 0.035728 |
| Atp5l | ATP synthase, H + transporting, mitochondrial F0 complex, subunit G (Complex V) | 1.1060036 | 0.019063 |
| Transcription, translation and metabolism associated genes | |||
| Mttp | Microsomal triglyceride transfer protein | 1.7323127 | 0.015456 |
| Fads6 | Fatty acid desaturase domain family, member 6 | 1.5089704 | 0.0251 |
| Pank1 | Pantothenate kinase 1 | 1.2655075 | 0.044121 |
| Nfyb | Nuclear transcription factor-Y beta | 1.2637761 | 0.016081 |
| Polr2f | Polymerase (RNA) II (DNA directed) polypeptide F | 1.2126642 | 0.038375 |
| Apoo | Apolipoprotein O | 1.2104626 | 0.015709 |
| Tceal1 | Transcription elongation factor A (SII)-like 1 | 1.163035 | 0.013797 |
| Cyp2d4 | Cytochrome P450, family 2, subfamily d, polypeptide 4 | 1.1584259 | 0.02751 |
| Eif4e | Eukaryotic translation initiation factor 4E | 1.142376 | 0.030993 |
| Oxidative stress related genes | |||
| Gsta5 | Glutathione S-transferase Yc2 subunit | 2.4397118 | 0.013714 |
| Downregulated | |||
| Mitocondrial associated genes | |||
| Mtfr1 | Mitochondrial fission regulator 1 | 1.2446603 | 0.047542 |
| Ptcd3 | Pentatricopeptide repeat domain 3 | 1.2458737 | 0.047166 |
| Inpp5b | Inositol polyphosphate-5-phosphatase B | 1.1909425 | 0.021694 |
| Mpv17l2 | MPV17 mitochondrial membrane protein-like 2 | 1.170515 | 0.012484 |
| Bckdha | Branched chain ketoacid dehydrogenase E1, alpha polypeptide | 1.1625147 | 0.010577 |
| Pitrm1 | Pitrilysin metallopeptidase 1 | 1.1204993 | 0.034489 |
| Gatm | glycine amidinotransferase (L-arginine:glycine amidinotransferase) | 1.1167421 | 0.013758 |
| Transcription, translation and metabolism associated genes | |||
| Gcgr | Glucagon receptor | 1.7509662 | 0.013298 |
| Glp2r | Glucagon-like peptide 2 receptor | 1.3274169 | 0.010548 |
| Stat6 | Signal transducer and activator of transcription 6 | 1.2245467 | 0.041221 |
| Oxidative stress related genes | |||
| Nrf1 | Nuclear respiratory factor 1 | 1.1174673 | 0.039902 |
Selection of relevant genes from the Agilent Microarray analysis from animals fed with KD compared to CD fed animals. Table shows the mitochondrial related genes; transcription, translational metabolism genes; and oxidative stress associated genes for which expression significantly changed (p < 0.05) (with the exception of Coq3 that with a p = 0.0554 was included in the selection as the gene we believe is relevant for the study).