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. 2021 Aug 11;12:4853. doi: 10.1038/s41467-021-24898-9

Fig. 3. PAK3 is a downstream effector of Smad4 mediating lung cancer cell metastasis.

Fig. 3

a Gene expression profiles were detected between PK and SPK cells by RNA-sequencing(RNA-seq) analysis. b KEGG Analysis of 3777 DEGs. c Analysis of DEGs overlapped between cancer and cell motility KEGG pathways. d Representative IHC images of PAK3 expression in PK and SPK mouse lung tumors. Scale bar, 50 μm (magnification, ×20). e Quantitative analysis of PAK3 RNA expression in PK and SPK mouse lung tumors. Each spot represents a sample from an individual mouse. n = 20, data represent means ± SEM; p < 0.001, as determined by two-tailed Student’s t-test. f Western blots showing decreased protein levels of PAK3 in shPAK3 (#1/#2) cells, and the experiment was repeated three times independently with similar results. g The migration ability of SPK shN and shPAK3 (#1/#2) cells were tested by wound healing assay. Cells were made a wound between the two dashed lines, the area of the two dashed lines represents the level of wound healing and the cell migration activity. Scale bar, 100 μm. h Statistical analyses of wound closure percentage. Data presented are the mean ± SEM from three biologically independent samples (n = 3); *p = 0.000360664; **p = 0.000524113; as determined by two-tailed Student’s t-test.