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. 2021 Aug 11;13:26. doi: 10.1038/s41368-021-00130-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Knockdown of METTL13 attenuates cell proliferation in vitro. a Western blot analysis of METTL13 expression in different HNSCC cells. GAPDH was served as internal control. b, c Knockdown efficiency of METTL13 in SCC9 and SCC15 cells was confirmed by western blot and real-time PCR analysis. d The colony formation assay was performed on SCC9 and SCC15 cells transfected with LV-shRNA-1, LV-shRNA-2, and LV-shCtrl, colony numbers were measured after crystal violet staining. e The CCK-8 assay examining the effect of METTL13 decrease on the proliferation of SCC9 and SCC15 cells. The experiments were performed in triplicate. f Cell apoptosis rate of SCC9 and SCC15 cells stable silenced METTL13 expression was detected by flow cytometry. Ratio of early and late apoptotic cells was collected and presented in the column chart. g Knockdown of METTL13 decreased cell migration capacity, as determined by the wound-healing assay. Scale bar, 100 μm. h Cell migration abilities of SCC9 and SCC15 cells stably transfected with vector or shRNA were evaluated by transwell migration assay. Scale bar, 100 μm