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. 2021 Aug 11;11:16323. doi: 10.1038/s41598-021-94933-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular characteristics of corneal endothelial cells (CenC). (a) Violin plots of cluster marker gene expression. SCANPY 1.7.1 in Python was used to generate the violin plots as indicted in the "Materials and methods" (https://pypi.org/project/scanpy/). (b) RNAScope on human cornea of CenC specific genes CA3, SLC4A11, and RGS5 (scale bar: 50 uM). Insert of zoomed in selection of endothelium is outlined for SLC4A11 and RGS5. (c) Topmost enriched GO terms (Biological Process) of genes up-regulated in CenC compared to other clusters. The number of overlap genes are indicated next to each bar.