Table 1.

Inhibitory activity of SC-919 on human IP6K1, human IP6K2, and human IP6K3 kinase activity.

Test material	IC50 (nmol/L)
	IP6K1	IP6K2	IP6K3
SC-919	<5.2a	<3.8a	0.65 ± 0.13
TNP	270 ± 100	850 ± 330	260 ± 21

The ADP-glo assay condition was performed as follow: The compounds were co-incubated with recombinant human IP6K1 (15 nM), human IP6K2 (12 nM), or human IP6K3 (1.5 nM) in the assay buffer. Following the addition of ATP and InsP₆ at final concentrations of 15 and 50 μM, respectively, a kinase reaction was carried out for 2 h. Thereafter, the ADP-Glo™ kinase assay (Promega, Madison, WI, USA) was performed.

^aSC-919 showed inhibitory activity reaching detection limits for IP6K1 assay and IP6K2 assay, respectively. Values indicate mean ± S.D. (n = 3 biological replicates of 4–8 technical replicates).