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. 2021 Aug 11;12(8):785. doi: 10.1038/s41419-021-04078-9

Fig. 4. Disulfiram induces decrease of H3K4 methylation.

Fig. 4

A–E Top: whole-cell lysates were prepared from the indicated cell lines, cultured in the absence or presence of 0.5 mM auranofin with increasing concentrations of disulfiram for 16 h. Extracts were probed with α-H3K4me2, α-H3K4me3, α-H3K9me2/3 antibodies, and α-H3 antibody as loading control. Bottom: the graphs represent image analysis of band intensity. Data are mean ± STD; n = 3; ∗p < 0.05, ∗∗p < 0.01 ∗∗∗p < 0.001; two-way analysis of variance (ANOVA) test with Bonferroni posttest. F Chromatin immunoprecipitation analysis of H3K4me2 enrichment at promoter regions of MYC and PAX2. ChIP was performed using chromatin from SF188 left untreated or treated for 16 h with 0.5 µM disulfiram. Data are mean ± STD; n = 3; ∗∗p < 0.01, student t-test.