FIGURE 3.

(A) After 24 hours apoptosis induction was quantified in PEITC-treated CaSki cells by Annexin V-FITC kit using flow cytometry. (B) Apoptosis percentage in PEITC-treated CaSki cells was measured. CaSki cells were induced with different concentration of PEITC (20, 25 and 30 μM) for 24 h and fluorescent microscopic images of Annexin V-FITC/PI stained cells were captured to examine the induction of apoptosis in CaSki cells under fluorescent microscope. PI can bind with damaged nucleus of cancer cells and Annexin V-FITC binds with phosphatidylserine of the plasma membrane. (C and D) Control untreated CaSki cells appear non-fluorescent while the fluorescent bright green and red demonstrate induction of apoptosis in CaSki cells. The data represents mean ±SD of three independent experiments. The image is the representation of three independent experiments.