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. 2021 Jul 29;9:652292. doi: 10.3389/fcell.2021.652292

FIGURE 2.

FIGURE 2

MiR-1-3p inhibited the proliferation, migration, and invasion and promoted apoptosis of HCC cells. (A) The transfection efficiency of miR-1-3p mimics and miR-1-3p inhibitors was examined using qRT-PCR. (B) After the transfection, the proliferation of Huh7 and Hep3B cells was detected utilizing the CCK-8 assay. (C) After the transfection, EdU assay was performed to determine the proliferation of Huh7 and Hep3B cells. (D) After the transfection, flow cytometry was performed to analyze the cell cycle distribution of Huh7 and Hep3B cells. (E) After the transfection, apoptosis of Huh7 and Hep3B cells was measured using flow cytometry. (F) After the transfection, migration and invasion of Huh7 and Hep3B cells were measured through Transwell assay. (G) After the transfection, Western blot was used to detect the expressions of Bax, PCNA, MMP-2, and MMP-9 of Huh7 and Hep3B cells. *, **, *** represent p < 0.05, p < 0.01, and p < 0.001, respectively.