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. 2021 Jul 29;9:652292. doi: 10.3389/fcell.2021.652292

FIGURE 5.

FIGURE 5

MiR-1-3p/ORC6 axis regulates the malignant biological behaviors of Huh7 cells. Huh7 cells were transfected with miR-1-3p mimics, or miR-1-3p mimics + empty vector, or miR-1-3p mimics + ORC6 overexpression plasmids, respectively. (A) The relative expression of miR-1-3p was detected with qRT-PCR. (B) The relative protein expression level of ORC6 was measured using Western blot. (C) CCK-8 assay was conducted to detect Huh7 cell proliferation. (D) EdU assay was performed to determine the percentage of cells in the S phase. (E) Flow cytometry was performed to analyze Huh7 cell cycle distribution. (F) Apoptosis of Huh7 cells was examined by flow cytometry. (G) Huh7 cell migration and invasion were measured using Transwell assay. (H) Western blot was used to detect the expression of Bax, PCNA, MMP-2, and MMP-9 of Huh7 and Hep3B cells. All of the experiments were performed in triplicate. *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001, respectively.