Table 2.
Effect of different storage temperatures and duration on in vitro pollen viability of four autochthon apple cultivars.
| Cultivars | Temperature | 0 month of storage | 1 month of storage | 2 month of storage | 3 month of storage | 4 month of storage | 5 month of storage | 6 month of storage | Anova P-Value |
|---|---|---|---|---|---|---|---|---|---|
| Petrovača | +20°C | 79 ± 6.1b/A | 34 ± 3.0f/B | 22 ± 1.9f/C | 18 ± 1.6g/D | 15 ± 2.4h/E | 11 ± 1.4k/F | 9 ± 1.0i/G | ≤0.0001 |
| +4°C | 79 ± 6.1b/A | 65 ± 5.4c/B | 30 ± 2.7e/C | 28 ± 2.4e/C | 26 ± 2.3f/D | 22 ± 2.5h/E | 19 ± 2.1f/E | ≤0.0001 | |
| −20°C | 79 ± 6.1b/A | 74 ± 7.8b/A | 70 ± 3.8b/B | 68 ± 3.6b/B | 66 ± 3.1b/B | 62 ± 3.1c/C | 60 ± 4.3b/C | ≤0.0001 | |
| −80°C | 79 ± 6.1b/A | 75 ± 6.4b/A | 72 ± 5.1b/A | 70 ± 4.2b/AB | 69 ± 4.0b/AB | 67 ± 3.0b/AB | 63 ± 3.3b/B | ≤0.0001 | |
| Budimka | +20°C | 98 ±8.7a/A | 55 ± 5.3d/B | 30 ± 2.4e/C | 24 ± 2.6f/D | 20 ± 2.1g/E | 16 ± 1.9i/F | 12 ± 1.4g/G | ≤0.0001 |
| +4°C | 98 ± 8.7a/A | 72 ± 7.3b/B | 56 ± 3.2cd/C | 48 ± 4.5d/D | 35 ± 3.1e/E | 30 ± 3.4g/F | 28 ± 2.9e/F | ≤0.0001 | |
| −20°C | 98 ± 8.7a/A | 95 ± 9.7a/A | 90 ± 8.9a/A | 86 ± 6.8a/AB | 80 ± 5.2ab/B | 79 ± 6.2a/B | 73 ± 7.6a/B | ≤0.0001 | |
| −80°C | 98 ± 8.7a/A | 96 ± 8.9a/A | 93 ± 7.7a/A | 90 ± 5.3a/A | 86 ± 3.7a/AB | 81 ± 3.5a/B | 79 ± 4.1a/B | ≤0.0001 | |
| Kolačara pozna | +20°C | 68 ± 6.3c/A | 41 ± 4.3e/B | 17 ± 1.5g/C | 13 ± 1.4h/D | 10 ± 1.4i/E | 9 ± 0.9k/F | 7 ± 0.8j/G | ≤0.0001 |
| +4°C | 68 ± 6.3c/A | 46 ± 4.5e/B | 30 ± 2.5e/C | 21 ± 2.3f/D | 15 ± 1.6h/E | 14 ± 1.5j/EF | 13 ± 1.5g/F | ≤0.0001 | |
| −20°C | 68 ± 6.3c/A | 65 ± 5.6c/A | 60 ± 5.0c/AB | 58 ± 3.2c/AB | 57 ± 5.6c/AB | 56 ± 3.8d/AB | 51 ± 3.4c/B | ≤0.0001 | |
| −80°C | 68 ± 6.3c/A | 66 ± 6.7c/A | 62 ± 5.8c/A | 59 ± 3.1c/AB | 58 ± 3.9c/AB | 57 ± 3.9d/B | 56 ± 3.7c/B | ≤0.0001 | |
| Kožara | +20°C | 59 ± 5.6d/A | 37 ± 3.9f/B | 12 ± 1.3h/C | 9 ± 0.8i/D | 7 ± 0.9j/E | 6 ± 0.7l/F | 5 ± 5.1k/G | ≤0.0001 |
| +4°C | 59 ± 5.6d/A | 40 ± 4.1e/B | 33 ± 3.1e/C | 28 ± 2.5e/D | 17 ± 1.8h/E | 14 ± 1.6j/F | 11 ± 1.2g/G | ≤0.0001 | |
| −20°C | 59 ± 5.6d/A | 56 ± 5.7d/A | 52 ± 4.7d/AB | 49 ± 3.2d/AB | 48 ± 2.7d/AB | 44 ± 3.7f/B | 40 ± 4.2 d/B | ≤0.0001 | |
| −80°C | 59 ± 5.6d/A | 58 ± 6.0d/A | 57 ± 3.2cd/A | 54 ± 3.1cd/A | 50 ± 3.8d/B | 49 ± 3.1e/B | 44 ± 4.6d/C | ≤0.0001 | |
| Anova P-value | ≤0.0001 | ≤0.0001 | ≤0.0001 | ≤0.0001 | ≤0.0001 | ≤0.0001 | ≤0.0001 |
Means followed by the same lower case letters in a column and capital letters in a row are not significantly different by the LSD test (p ≤ 0.05).