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. 2021 Jul 29;9:693782. doi: 10.3389/fcell.2021.693782

FIGURE 5.

FIGURE 5

Adipose-derived mesenchymal stem cells alleviated radiation-induced oxidative stress and inflammation, and inhibited the expression of CD68 in microglia. Microglia were pretreated with exosomes (2.0, 4.0, or 8.0 μg) 15 min before irradiation. Expressions of NO (A), SOD (B), TNF-α (C), and IL-10 (D) at 24 h after irradiation measured by ELISA (n = 6) (*p < 0.05, compared with the Ctr; #p < 0.05, compared with the RT; ^p < 0.05, compared with the Exo 2.0 μg; △ p < 0.05, compared with the Exo 4.0 μg). (E) Microglia were stained by CD68 (green) and IBA1 (red), the nuclei were stained by DAPI (blue) (scale bar = 20 μm). EX527 abolished the effect of ADMSC-Exo on the expression of CD68 in microglia. (F) Fluorescent density of CD68 at 24 h after irradiation (n = 6). (G) Expression of CD68 at 24 h after irradiation determined by Western blot (n = 3) (*p < 0.05, compared with the Ctr; #p < 0.05, compared with the RT;^p < 0.05, compared with the Exo).