Fig. 2.
Changes in Se status as well as related biomarkers and genes. Markers for the evaluation of the Se status were determined in serum and liver of adult (30 weeks) and old (66 weeks) male and female C57BL/6Jrj mice (n = 8–10) receiving a -TE, +TE, or +TEaa diet for 26 weeks. Se concentrations were analysed in serum (A) and liver (D) by ICP-MS/MS. To further evaluate the Se status, GPX enzyme activity was analysed by a NADPH-consuming assay in serum (B) and liver (E). Additionally, serum Selenop concentrations (C) were analysed by affinity-HPLC-ICP-MS/MS and hepatic Selenop transcription level was examined by qRT-PCR analysis, whereby hepatic transcription levels were normalised to a composite factor based on the house keeper genes Hprt and Rpl13a. Variances are expressed as fold changes compared to +TE male adults (mean +TE male adult = 1). Statistical testing based on Three-Way ANOVA and Bonferroni's post-test with *p < 0.05, **p < 0.01, ***p < 0.001. Detailed results of statistical testing are summarised in Table 2.