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. Author manuscript; available in PMC: 2022 Oct 10.
Published in final edited form as: Cancer Lett. 2021 Jul 14;518:152–168. doi: 10.1016/j.canlet.2021.07.015

Figure 2: Stable overexpression of HNRNPA2B1 (A2B1) in MCF-7 cells.

Figure 2:

A2B1 transcript (A) and protein (B and C) levels were measured in the G418-selected MCF-7-A2B1 cells and MCF-7 cells. GAPDH and α-tubulin were was used as a normalization controls for qPCR and western blots respectively. C) Summary of 16 separate western blots, each with 3–8 separate preparations of WCE. *p < 0.0001, Student’s two tailed t-test. Phase contrast images of MCF-7 (D) and MCF-7-A2B1 cells (E) at 20X phase contrast imaging, scale bar = 100 μm (additional images in Supplementary Figure 4). F) Representative western blot showing E-cadherin and vimentin in WCE from the indicated cell lines. Quantification is relative to Ponceau S staining with the MCF-7 sample in the first lane set to 1.0 for relative quantification.