FIGURE 3.

Definition of the murine plasmablast‐specific signature. (a) Workflow defining the different clusters according to their gene expression patterns. The 155 genes dispatched in the 4 clusters characterize the plasmablast (PB)‐specific gene expression compared with early B cells and plasma cells (PC), respectively. The clusters A and B described genes with a continuous up‐ or down‐regulation during the B‐cell differentiation (transitional expression level in PB). The clusters C and D described genes with a peak of up‐regulation or down‐regulation at the PB stage (specific expression level in PB). (b) Heatmaps of the median fold change (FC) of the differential expressed genes (DEGs) in PB versus early B cells and in PC versus PB. Each gene is classed in the four different clusters. The genes mentioned in text are coloured in red when up‐regulated and blue when down‐regulated genes in PB. Row min and row max are minimal and maximal values for each row. (c) Gene ontology analysis of the genes with transitional expression level in PB (clusters A and B) or with a specific expression level in PB (clusters C and D)