FIGURE 4.

CD11c deficiency could impair the ability of antigen presentation function of DCs. (a) Representative flow cytometric analysis of the percentage of CD11b⁺, MHCII⁺, CD40⁺ and CD80⁺ in CD45⁺ cells in spleen in CD11c^−/− mice or WT mice. (b) Percentages of cells expressing CD11b, MHCII, CD40, CD80 and CD86 in CD45⁺ cells in spleen in CD11c^−/− mice or WT mice (n = 3). (c) Relative MFI (mean fluorescence intensity) of CD11b, MHCII, CD40, CD80 and CD86 in respective positive cells in spleen in CD11c^−/− mice or WT mice (n = 3). (d) Representative flow cytometric analysis of CFSE‐labelled CD4⁺ T cells sorted from OT‐II transgenic mice cocultured with BMDCs from CD11c^−/− mice or WT mice for 3 days pretreated with OVA for 30 min. (e) Percentages of proliferative CD4⁺ T cells sorted from OT‐II transgenic mice cocultured with BMDCs from CD11c^−/− mice or WT mice for 3 days. Results are presented as mean ± SD, *P < 0·05, Student's t‐test (b, c, e). All data are representative of two‐three independent experiments