FIGURE 2.

Neutralization of IL‐22 exacerbates experimental autoimmune encephalomyelitis (EAE) disease scores (a) After EAE was induced by MOG_35‐55 immunization, one group of mice received every other day 150 µg anti‐mouse IL‐22, and the other group received isotype antibody until day 10 post‐immunization. The EAE was scored daily. (b) On day 22, spinal cords were removed and stained with Luxol fast blue and scored to quantify demyelination (n = 9–10 per group). (c) Spinal cords and brain tissues from anti‐IL‐22 and isotype antibody (control) groups were harvested 3 weeks after EAE induction, and infiltrating lymphocytes were quantified based on IFN‐γ, IL‐17A and GM‐CSF production. Absolute number or percentages of indicated cytokine‐producing CD4⁺ T cells were quantified (n = 9–10 per group). The experiments were repeated 3 times. (*) indicates p‐value <0·05