FIGURE 6.

Reg3γ overexpression causes dysbiosis in the intestinal microbiota in experimental autoimmune encephalomyelitis (EAE) mice (a) Reg3γ plasmid or empty vector was injected on day −2, and EAE was induced by MOG_35‐55 immunization on day 0. Mice were killed on day 20, and ileal faeces were collected for microbiota analyses. Principal co‐ordinate analysis ordinated based on the Unweighted UniFrac beta‐diversity metric plot (n = 7 per group). (b) Genus‐ and phylum‐level abundance profile of ileal microbiota in Reg3γ plasmid‐ or empty vector‐injected EAE mice at the peak of the disease (n = 7 per group). (c) Total microbiota abundance of ileal microbiota in Reg3γ plasmid‐ or empty vector (control)‐injected EAE mice at the peak of the disease (n = 7 per group), p‐value <0·05. (d) Significantly reduced genera of microbes in ileal microbiota of Reg3γ plasmid‐injected compared with empty vector‐injected EAE mice at the peak of the disease (n = 7 per group). All genera plots shown in ‘D’ are significantly altered (p‐value <0·05). (e) Significantly enriched (all plots shown in ‘E’ have p‐value <0·05) genera of microbes in ileal microbiota in Reg3γ plasmid‐injected compared with empty vector‐injected EAE mice at the peak of the disease (n = 7 per group), (*) indicates p‐value <0·05