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. 2021 Aug 8;12:2040622321997259. doi: 10.1177/2040622321997259

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© The Author(s), 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — KDM4C/MALAT1/miR-328-3p/CCND2 axis regulates in vivo Ara-C resistance in AML. (a) RT-qPCR to detect the expression of KDM4C, MALAT1, miR-328-3p, and CCND2 in corresponding stably transfected HL-60/A cells, *p < 0.05 compared with sh-NC + oe-NC, ^#p < 0.05 compared with sh-KDM4C + oe-NC. (b) Western blot analysis to detect the expression of KDM4C, MALAT1, miR-328-3p, and CCND2 in NOD/SCID mice under light microscope (left); statistical chart of Western blot analysis (right), *p < 0.05 compared with HL-60, # p < 0.05 compared with HL-60/A sh-NC + oe-NC, & HL-60/A sh-KDM4C + oe-CCND2. (c) The survival of mice in each group, *p < 0.05 compared with HL-60/HL-60/A sh-NC + oe-NC/HL-60/A sh-KDM4C + oe-CCND2. (d) The quantification of the ratio of leukocytes, *p < 0.05 compared with HL-60/HL-60/A sh-NC + oe-NC/HL-60/A sh-KDM4C + oe-CCND2. (e) H&E staining to show the lesion and tumor infiltration in liver tissues of grouped mice under light microscope, × 200. (f) H&E staining to show the lesion and tumor infiltration in spleen tissues of grouped mice under light microscope, × 400. Experiments were repeated three times. Data among multiple groups were analyzed by one-way analysis of variance (ANOVA). Data at different time points among multiple groups were analyzed by repeated measures ANOVA. p < 0.05 indicated statistically significant difference.

AML, acute myeloid leukemia; Ara-C, cytarabine; H&E, hematoxylin and eosin; NC, negative control; oe, overexpression; RTqPCR, real-time quantitative polymerase chain reaction; sh, short hairpin; miR-328-3p, microRNA-328-3p.