Figure 1.

Chimeric RabMab1 antibodies have asTF binding affinities comparable to rRabMab1. Two human-rabbit chimeric RabMab1 antibodies were compared to parental rRabMab1 for their ability to bind asTF. (A) Serial dilutions of recombinant or chimeric RabMab1 were added to asTF-coated ELISA plates. Anti-human or anti-rabbit HRP conjugated secondary antibodies were used to assess antibody binding, hFc = non-targeting human Fc fragment. (B) HIS1K biosensor assessment of asTF binding kinetics of each antibody. (C) Trace results of AHC biosensor assessment of chimeric antibodies. (D) asTF binding kinetics as assessed by AHC biosensor experiments.