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. 2021 Jul 29;12:719810. doi: 10.3389/fimmu.2021.719810

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Copyright © 2021 Moström, Scheef, Sprehe, Szeltner, Tran, Hennebold, Roberts, Maness, Fahlberg and Kaur

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NK cell composition in PBMC and decidua of normal rhesus macaque pregnancies. (A) Representative flow plots of NK cells CD16/CD56 subsets. (B) NK cell subset frequencies in PBMC and decidua first (yellow), second (red) and third trimester (blue) of normal pregnant rhesus macaques. (C) NKG2A/NKG2D flow plots with representative gating of PBMC and decidua. (D) Frequency of NKG2A+NKG2D+ and NKG2A-NKG2D+ NK cells in PBMC and decidua. (E) Granzyme B expression frequency and MFI on NK cell subsets. (F) CD56- vs CD56+ NK cell expression of CXCR3, CX3CR1, CD69 and the CD169 on total NK cells. (G) Representative flow plot of dNK1, dNK2, and dNK3 subsets with box plots showing their frequency in PBMC and decidua. P-values < 0.05 (unpaired t-test) shown on the plots.