Table 2.
Flow cytometric definition of cell types.
| Cell type* | Definition | Panel |
|---|---|---|
| αβ T cells | CD3+ panγδ– | Adaptive 28-color |
| CD4+: CD4+ CD8– | ||
| CD8+: CD4– CD8+ | ||
| Double Positive (DP+): CD4+ CD8+ | ||
| Double Negative (DN–): CD4– CD8– | ||
| γδ T cells | CD3+ panγδ+ | Adaptive 28-color |
| Vγ9+ Vδ2+ | ||
| Vγ9+ Vδ2– | ||
| Vγ9– Vδ2+ | ||
| Vγ9– Vδ2– | ||
| iNKT | CD3+ Vα24+ CD1d tetramer+ | Adaptive 28-color |
| T regulatory cells | CD3+ CD4+ CD127– CD25hi | Adaptive 28-color |
| Memory T cells | Naïve: CD95– CD28+ CD45RA+ | Adaptive 28-color |
| In CD4+ αβ T | Total Memory: CD95+ CD28+/– | |
| In CD8+ αβ T | Central Memory (CM): CD95+ CD28+ CCR5– | |
| In γδ T | Transitional Effector Memory (Tem): CD95+ CD28+ CCR5+ | |
| Effector Memory (EM): CD95+ CD28– CD45RA– | ||
| Terminal Effector Memory (TEMRA): CD95+ CD28– CD45RA+ | ||
| Tissue Resident Memory (Trm): CD95+ CD28+/– CD103+ CD69+ | ||
| T helper subsets | CD3+ CD4+ | Adaptive 28-color |
| CXCR3+ CCR5+/– Th1-like | ||
| CCR4+ Th2-like | ||
| CCR6+ Th17-like | ||
| NK cells | CD3– HLA-DRlo/- CD8+ | Innate 18-color and adaptive 28-color |
| CD16+ CD56– | ||
| CD16– CD56+ | ||
| CD16+ CD56+ | ||
| CD16– CD56– | ||
| B cells | CD3– HLA-DRhi CD20+ | Adaptive 28-color |
| Macrophages in Decidua | CD3– CD20– HLA-DR+ CD8– CD14+ | Innate 18-color |
| CD163+ CD206+ | ||
| CD163+ CD206– | ||
| CD163– CD206– | ||
| Monocytes in PBMC | CD3– CD20– HLA-DR+ CD8– | Innate 18-color |
| Classical monocytes: CD14+ CD16– | ||
| Intermediate monocytes: CD14+ CD16+ | ||
| Non-classical monocytes and DCs: CD14– CD16+ | ||
| Dendritic cells (DC) | CD3– CD20– HLA-DR+ CD14– | Innate 18-color |
| Conventional DC, myeloid DC, and non-classical monocytes: CD123– | ||
| Plasmacytoid DC: CD123+ |
*Cells are pre-gated as Live+ Single CD45+ Leukocytes.