Skip to main content
. 2021 Jul 6;25(16):7840–7854. doi: 10.1111/jcmm.16698

FIGURE 7.

FIGURE 7

Enhanced glycolysis in muscle regulated systemic homeostasis through endocrine actions of FGF21. (A) mRNA expression of indicated genes in the M;G and control mice fed on HFD. n = 6 control mice, n = 5 M;G mice. (B) Plasma FGF21 levels in the mice fed on HFD for 5 months. n = 4 control mice, n = 6 M;G mice. (C) mRNA expression of indicated genes in eWAT of the HFD‐fed mice. n = 6 control mice, n = 5 M;G mice. (D) Western blot analysis of ChREBP and Mlx in GC muscle from the mice fed on HFD. (E) G‐6‐P levels in EDL muscle of the HFD‐fed mice. n = 6. (F) Western blot analysis of the 3Genes‐OE C2C12 myotubes. (G) Cellular G‐6‐P levels in the 3Genes‐OE myotubes under the treatment of doxycycline. n = 3. (H) FGF21 levels in CM from the indicated myotubes. n = 3. (I) Western blot analysis of the 3Genes‐OE myotubes transfected with either ChREBP‐specific or control siRNA, with or without the treatment of doxycycline. FAM‐control indicates fluorescein amidites‐labelled control siRNA. (J) Western blot analysis of the indicated C2C12 myotubes under the treatment of doxycycline. (K) mRNA expression of indicated genes in the primary visceral white adipocytes treated with either control or 3Genes‐OE CM, with or without the treatment of anti‐FGF21 antibody. n = 3. Values are means ± SEMs, *P < .05; **P < .01; ***P < .001 (t test).