FIGURE 4.

A, Percentage of patients with expression of Collagen Type I (COL‐I) and Collagen Type III (COL‐III) with Sirius Red technique. (orange‐red = COL‐I, yellow‐green = COL‐III). B‐E, Images of histological staining of collagen fibres with Sirius red in the venous wall of the different study groups. NR<50, patients younger than 50 y without reflux; NR≥50, patients without reflux aged 50 y or older; R < 50, patients younger than 50 y with reflux; R ≥ 50, patients with reflux aged 50 y or older; I, tunica intima; M, tunica media; A, tunica adventitia. NR (n = 29) [NR<50 (n = 13); NR≥50 (n = 16)]; R (n = 81) [R < 50 (n = 32); R ≥ 50 (n = 49)]. For statistical analyses, was used Mann‐Whitney U test