FIGURE 6.

Nrf2 inhibits NLRP3 inflammasome activation in the C. albicans and Leuk1 co‐culture model. Nrf2 was silenced (LV‐Nrf2 group) or activated (SFN group) in CSE‐treated co‐cultured cells. A, NLRP3 and caspase‐12 expression levels were assessed by Western blotting. B, Densitometry of Western blots. GAPDH was used as a loading control. Graphs show the densitometric analysis (protein/GAPDH) of each band. C, Cytokine levels (IL‐β and IL‐18) in supernatant were measured using ELISA kits. N = 3. Each bar represents the mean ± SD. *P < .05, **P < .01, compared between two groups. CSE: cigarette smoke extract; Candida albicans: Candida albicans; control: CSE‐treated C. albicans and Leuk1 co‐culture group; SFN: sulphoraphane; Casp1 p20: active form of caspase‐12