FIGURE 5.

The effect of PRMT1 on the methylation of STAT3 and FAS transcription. U‐2 OS cells transfected with pLenti control or pLenti‐PRMT1 plasmids were administrated with 5 µM furamidine dihydrochloride (DB75), a PRMT1 inhibitor, for 8 h. A, The expression of PRMT1 was detected by Western blot. B, Methylation of STAT3 was tested by immunoprecipitation with anti‐mono and dimethyl‐arginine antibody (MetR) and immunoblotting with an antibody against STAT3. C, The phosphorylation of STAT3 was tested in nucleus by Western blot. D, The mRNA level of FAS was detected by qPCR assay. The mRNA analysis result is expressed as the fold increase over control. n = 3. *P < 0.05 vs the Con group. **P < 0.01 vs the Con group