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. 2021 Aug 15;207(4):1033–1043. doi: 10.4049/jimmunol.2001381

FIGURE 3.

FIGURE 3.

cTfh cells and germinal center activity following YFV 17D vaccination. (A) Gating strategy of cTfh cells defined as CXCR5+ CD4+ T cells with Th1-polarized CXCR3+ and Th2/17-polarized CXCR3− subsets. (B) Total cTfh, (C) Th1-polarized CXCR3+ cTfh, and (D) CXCR3− cTfh cell frequencies of CD4+ T cells over time following vaccination. (E) Representative gating of frequencies of Th1-polarized CXCR3+ cTfh cells expressing CCR7, Ki67, ICOS, and PD1. (F) Expression of CCR7 and activation of Th1-polarized CXCR3+ cTfh cells over time as assessed by dual expression of (G) PD1 and Ki67, (H) ICOS and Ki67, and (I) PD1 and ICOS. Comparison of expression of CCR7, Ki67, ICOS, and PD1 between Th1-polarized CXCR3+ (green) and Th2/17-polarized CXCR3− (blue) cTfh cells on (J) day 7 and (K) day 14 following vaccination. (L) Change in germinal center activity over time as defined by serum CXCL13 concentrations determined by ELISA (n = 10). All graphs plotted as median with 95% confidence interval (CI) (n = 8 unless otherwise stated). Statistical analysis was performed using nonparametric Friedman test and corrected for multiple comparisons with Dunn multiple comparison test (B–D, F–I, and L) or nonparametric Wilcoxon matched-pairs signed-rank test. *p < 0.05, **p < 0.01.