Figure 3. Knockdown of BRG1 inhibits tubular senescence and the activation of Wnt/β-catenin signalling pathway in UUO mice.

(A–D) Western blot analysis demonstrated that knockdown of BRG1 attenuated renal expression of p16^INK4a, p21, and p19^ARF proteins induced by UUO. Representative Western blot (A) and quantification of protein levels of renal p16^INK4a (B), p21 (C), and p19^ARF (D) are presented. (E) Representative staining micrographs showed renal SA-β-gal activity, p16^INK4a, and TGF-β1 expression in different groups as indicated. Frozen kidney sections were stained for SA-β-gal activity, paraffin-embedded kidney sections were immunostained with antibodies against p16^INK4a or TGF-β1. Scale bar, 50 μm. (F–J) Western blot analysis showed that renal expression of active-β-catenin, MMP-7, snail-1, and PAI-1 were significantly inhibited by knockdown of BRG1 in UUO mice. Representative western blot (F) and quantitative data (G–J) are presented. **P<0.01, ***P<0.001 versus sham group; ^#P<0.05, ^##P<0.01, ^###P<0.001 versus UUO alone (n=6).