FIGURE 7.

Vd424Y contributes to the virulence of Verticillium dahliae. (a) Images of Vd991 (wild type), ΔVd424Y mutant, and ΔVd424Y/VD424Y‐complementation transformants cultured on potato dextrose agar plates at 25 °C for 7 days in the dark. (b) Colony diameter of Vd991, ΔVd424Y mutant, and ΔVd424Y/VD424Y transformants. The data shows the mean across three independent replicates. (c) Images of disease symptoms on cotton 26 days postinoculation (dpi). The cotton cultivar Jimian 11 was inoculated with Vd991, ΔVd424Y mutant, and ΔVd424Y/VD424Y transformants. Images are representative of three independent experiments. (d) Disease index of the cotton plants at 26 dpi. The disease index (DI) was calculated as the following formula: DI = [(Ʃdisease grades × number of infected plants)/(total checked plants × 4)] × 100%. Seedlings were classified into five grades (0, 1, 2, 3, and 4). The data represent the mean across three independent experiments