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. 2021 Jun 24;22(9):1041–1056. doi: 10.1111/mpp.13093

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© 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Characterization of different GhSACPD genes via phylogenetic tree analysis and virus‐induced gene silencing (VIGS) experiment. (a) Phylogenetic tree of SACPD proteins from Gossypium hirsutum (Gh) and Arabidopsis thaliana (At) plants. The neighbour‐joining tree was generated using the MEGA 5 program. According to the phylogenetic tree, GhSACPDs were divided into groups I to VII. (b) Spontaneous lesion formation on the leaves of TRV:SACPD and TRV:1563 plants. TRV:3299, TRV:0927, TRV:1317, and TRV:1330 plants showed no difference compared with TRV:00. Photographs were taken 2 weeks after VIGS. (c) Detection of fatty acid content in the silenced cotton lines. The values are the mean ± SE for three biological replicates (**p < 0.01, Student's t test)