FIGURE 1.

N⁶ methylation of adenosine (m⁶A) level in rice black streaked dwarf virus (RBSDV)‐viruliferous small brown planthopper (SBPHs) was lower than that in nonviruliferous SBPHs. (a) m⁶A levels of nonviruliferous and viruliferous SBPHs detected by an EpiQuik m⁶A RNA Methylation Quantification Kit (Colorimetric). (b) Fluorescence of m⁶A and RBSDV in midgut epithelial cells of nonviruliferous and viruliferous SBPHs. Excised midguts were incubated with anti‐m⁶A (green label) and anti‐RBSDV (red label) antibodies and DyLight 633 phalloidin (blue), then observed with a confocal laser‐scanning microscope. Scale bars, 20 μm. (c) Relative intensity of m⁶A fluorescence. Ten midgut epithelial cells each from nonviruliferous SBPHs and viruliferous SBPHs were considered. Significant differences between mean values for relative intensity were determined by Student's t test using Prism 8 (**p < 0.01)