FIGURE 5.

Accumulation of superoxide under menadione treatment and in cotton roots inoculated with Vd991 and ΔSOD1. (a) Hyphae from wildtype strain Vd991, VdSOD1 deletion strain, and the complemented (EC) strain were grown in liquid complete medium for 4 days and treated with 5 μM menadione for 24 hr. Strains not treated with menadione were included as untreated control. H₂DCFDA was used as a fluorescent dye to visualize the production of H₂O₂. (b) Cotton roots inoculated with the wildtype strain, ΔSOD1, and complemented strains at 1 and 5 days postinoculation. The production of ${\mathrm{O}}_2^{‐}$ and H₂O₂ was visualized by p‐nitroblue tetrazolium (NBT) and 3,3′‐diaminobenzidine (DAB) staining, respectively