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. 2021 Aug 12;18:30. doi: 10.1186/s12989-021-00420-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — TEM analysis of MNPs@SiO₂(RITC)-treated BV2 cells. a Schematic representation of the MNPs@SiO₂(RITC) composition. b Immunostaining image of Iba1 (green), MNPs@SiO₂(RITC) (red), and the nucleus (blue). Scale bar = 20 μm. c Representative TEM images of MNPs@SiO₂(RITC)-treated BV2 cells. Magnified images are presented in the bottom panels. The red arrow head indicates MNPs@SiO₂(RITC) in the cell, and the yellow arrow head indicates a granular structure. Scale bar = 1 μm. d ICP-MS peak of the cobalt ion in the control and MNPs@SiO₂(RITC)-treated microglia. IS = internal standard. e Quantification of the cobalt concentration and MNPs@SiO₂(RITC) particles in each MNPs@SiO₂(RITC)-treated cell