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IgG and IgA antibody titers in serum (A, B), trachea mucus (C), bile (D), and Harderian gland (E) following administration of PBS i.m., O-2'-HACC i.m., pVAX I -F(o)-C3d6 i.m., live attenuated NDV vaccine i.n., O-2'-HACC/pFDNA i.m., O-2'-HACC/pFDNA i.n. Data were representative of three independent experiments and presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01
