. 2021 Aug 11;19:240. doi: 10.1186/s12951-021-00983-5

Table 1.

Analysis of lymphocyte proliferation in SPF chickens immunized with the O-2'-HACC/pFDNA i.n., O-2'-HACC/pFDNA i.m., pVAX I -F(o)-C3d6 i.m., live attenuated NDV vaccine i.m., O-2′-HACC i.m. and PBS i.m. groups

Groups	Weeks post the immunization
Groups	1	2	3	4	5	6	7	8	9	10
O-2'-HACC/pFDNA i.n	0.604 ± 0.014^a	2.490 ± 0.038^a	3.213 ± 0.018^a	4.141 ± 0.012^a	4.410 ± 0.011^a	4.384 ± 0.056^a	4.360 ± 0.045^a	3.930 ± 0.054^a	3.228 ± 0.042^a	2.983 ± 0.034^a
O-2'-HACC/pFDNA i.m	0.568 ± 0.003^a	2.350 ± 0.046^a	3.086 ± 0.047^b	3.705 ± 0.022^b	4.145 ± 0.110^b	3.756 ± 0.108^b	2.668 ± 0.032^b	2.345 ± 0.049^b	1.986 ± 0.019^b	1.639 ± 0.010^b
Live attenuated NDV vaccine i.m	0.674 ± 0.008^a	2.660 ± 0.015^a	3.368 ± 0.040^a	4.192 ± 0.016^a	4.488 ± 0.015^a	4.422 ± 0.012^a	4.439 ± 0.078^a	3.989 ± 0.014^a	3.162 ± 0.055^a	2.970 ± 0.031^a
pVAX I -F(o)-C3d6 i.m	0.435 ± 0.018^b	2.124 ± 0.006^b	2.779 ± 0.017^c	3.148 ± 0.047^c	3.598 ± 0.013^c	3.278 ± 0.017^c	2.440 ± 0.005^c	1.897 ± 0.009^c	1.810 ± 0.010^c	1.683 ± 0.011^b
O-2′-HACC i.m	0.402 ± 0.004^b	1.058 ± 0.060^c	1.968 ± 0.019^d	2.156 ± 0.011^d	2.256 ± 0.038^d	2.171 ± 0.012^d	2.144 ± 0.010^d	2.023 ± 0.040^d	1.972 ± 0.017^b	1.581 ± 0.037^c
PBS i.m	0.306 ± 0.010^c	1.301 ± 0.006^d	1.924 ± 0.017^d	2.085 ± 0.018^d	2.117 ± 0.007^e	1.905 ± 0.009^e	1.879 ± 0.019^e	1.839 ± 0.016^e	1.702 ± 0.008^d	1.459 ± 0.022^d

Values represent mean ± SD (n = 3). Values within the same column with the different lower case letters (a–e) in the superscript indicate statistically significant differences (P < 0.05)