Skip to main content
. 2021 Jan 6;47(5):625–639. doi: 10.1111/nan.12686

FIGURE 2.

FIGURE 2

Oligomeric Aβ increases RAPGEF2 levels. A, SDS‐PAGE analysis of oligomeric Aβ. Oligomers were separated by western blotting on a 4%–12% gradient Bis‐Tris gel and immunoblotted with the 6E10 antibody. Oligomeric Aβ (AβO) consisted of monomers (~4 kDa), dimers, trimers, tetramers and high molecular weight (MW) oligomers. No fibrils (>75 kDa) were detected. B, Cultured cortical neurons (DIV 21) treated with vehicle or AβO (1 μM) for 6 h and immunoblotted for RAPGEF2. C, Relative fold change in RAPGEF2 levels (n = 7). D, Representative RAPGEF2 fluorescence images of cultured hippocampal neurons. Neurons (DIV 21) treated with vehicle or oligomeric AβO (1 μM) for 6 h and immunolabelled for GFP and RAPGEF2. Scale, 100 μm. E, Quantification of the integrated intensity of RAPGEF2 in proximal dendrites (n = 12). All data are shown as the mean ± SEM. *p < 0.05; two‐tailed unpaired Student's t‐test