FIGURE 4.

Knockdown of RAPGEF2 decreases JNK activation in 3xTg‐AD mice. A, Lentiviral particles expressing either shRAPGEF2 or a control vector (pll3.7) were transduced into cultured cortical neurons at DIV 7. After 8 days, cell culture lysates from wild‐type (WT) and 3xTg‐AD (TG) mice were used for western blot analysis of RAPGEF2 and MAPK levels (n = 3). B–E, Quantification of RAPGEF2 (B), pJNK (C), pp38 (D), and pERK (E) levels. All data are shown as the mean ± SEM. *p < 0.05; Two‐way ANOVA, Tukey's multiple‐comparison test