FIGURE 7.

Knockdown of RAPGEF2 preserves excitatory synapses in 3xTg‐AD mice. A, Representative electron microscopic images of the CA1 stratum radiatum of wild‐type (WT) and 3xTg‐AD mice injected with either control or shRAPGEF2‐expressing viral particles. The arrow indicates postsynaptic density. Scale, 1 μm. B, Representative images of asymmetric (left) and symmetric (right) synapses. The arrow indicates postsynaptic density. Scale, 0.5 μm. C and D, Quantification of the number of asymmetric (C) and symmetric (D) synapses for each condition. The data are shown as the mean ± SEM (n = 95–97 images from three male mice per group). *p < 0.05, **p < 0.01; Two‐way ANOVA, Tukey's multiple‐comparison test