TABLE 1.
Comparison of molecular and morphological identification of ticks
| Sample identification | Morphological identification | 16S rRNA (% homology, GenBank accession) | ITS2 (% homology, GenBank accession) | CO1 (% homology, GenBank accession) | Consensus identification (GenBank accessions) |
|---|---|---|---|---|---|
| T15 | Rhipicephalus sp. | Rh. decoloratus (100, EU918193) | Boophilus decoloratus (96.7, U97716) | – | Rh. decoloratus (16S: MN266914; ITS2: MN266918) |
| T16 | Rh. appendiculatus | Rh. appendiculatus (99.35, KC503257) | Rh. appendiculatus (99.8, KX276951) | Rh. appendiculatus (100, KC503257) | Rh. appendiculatus (16S: MN266911; ITS2: MN266949; CO1: MN294736) |
| T34 | Rhipicephalus sp. | Rh. microplus (99.2, MH513311) | Rh. microplus (99.6, KC503265) | Rh. microplus (100, KY678120) | Rh. microplus (16S: MN264523; ITS2: MN266952; CO1: MN294738) |
| T50 | Rhipicephalus sp. | Rh. microplus (99.3, KY020993) | Rh. microplus (99.6, MG721035) | Rh. microplus (100, KY678120) | Rh. microplus (16S: MN264524; ITS2: MN266953; CO1: MN294739) |
| T62 | Rhipicephalus sp. | Rh. decoloratus (100, EU918193) | Boophilus decoloratus (96.7, U97716) | – | Rh. decoloratus (16S: MN266915; ITS2: MN266919) |
| T63 | Rhipicephalus sp. nymph | Rh. appendiculatus (99.35, KC503257) | Rh. appendiculatus (99.35, KC503257) | Rh. appendiculatus (99.9, KC503257) | Rh. appendiculatus (16S: MN266912; ITS2: MN266950; CO1: MN294737) |
| T105 | Rh. decoloratus | Rh. microplus (99.1, MH513311) | – | – | Rh. microplus (16S: MN264525) |
| T134 | Rh. decoloratus | Rh. decoloratus (100, EU918193) | Boophilus decoloratus (96.7, U97716) | – | Rh. decoloratus (16S: MN266916; ITS2: MN266921) |
| T192 | Haemaphysalis sp. | Ha. elliptica (95.6, HM068961) | Ha. erinacei (88, KU364288) | Ha. erinacei (99.3, KU880573) | Haemaphysalis sp. (16S: MN264214; ITS2: MN266944;CO1: MN294735) |
| T199 | Am. variegatum | Am. variegatum (99.3, L34312) | Am. variegatum (100, HQ856803) | – | Am. variegatum (16S: MN266929; ITS2: MN401349) |
| T218 | Rhipicephalus sp. nymph | Rh. appendiculatus (99.51, KC503257) | Rh. appendiculatus (99.73, KY457500) | – | Rh. appendiculatus (16S: MN266913; ITS2: MN266951) |
| T222 | Am. gemma | – | Am. hebraeum (99.65, KY457490) | – | Am. gemma (ITS2: MN401350) |
| T311 | Amblyomma sp. nymph | Am. variegatum (99.3, L34312) | Am. variegatum (100, HQ856803) | – | Am. variegatum (16S: MN266930; ITS2: MN401351) |
| T321 | Rhipicephalus sp. | Rh. simus (96.28, KJ613641) | – | – | Rhipicephalus sp. (16S: MN266945) |